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| Product Name: |
Human Cholesterol 7α-Hydroxylase , CYP7A ELISA Kit |
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| Product Type: | ELISA Kit | |
| Code: | CSB-E14344h | |
| Alias: | CP7A, CYP7, MGC126826, MGC138389, cholesterol 7 alpha-monooxygenase|cholesterol 7-alpha-hydroxylase|cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 | |
| Size: | 96T | |
| Species: | Human | |
| other species: | Mouse Rat | |
| Target Name: | cytochrome P450, family 7, subfamily A, polypeptide 1 | |
| Abbreviation: | CYP7A1 | |
| Protein Biological Process 1: | Biosynthesis/Metabolism | |
| Protein Biological Process 2: | Lipogenesis and lipometabolism | |
| Protein Biological Process 3: | Cholesterol metabolism | |
| Detect Range: | 18.75 pg/ml-1200 pg/ml | |
| Sensitivity: | 4.7 pg/ml | |
| Assay Time: | 1-5h | |
| Sample Volume: | 50-100ul | |
| Detection wavelengt: | 450 nm | |
| Specificty: | This assay has high sensitivity and excellent specificity for detection of Human CYP7A1. No significant cross-reactivity or interference between Human CYP7A1 and analogues was observed. | |
| Precision: | Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. |
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| Sample collection and storage: | Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. |
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| Assay procedure: | Bring all reagents and samples to room temperature before use. Centrifuge the sample again after tha...... | |
| Calculation of results: | Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be...... |
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