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Human Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial , ECH1 ELISA Kit
Human Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial , ECH1 ELISA Kit
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ELISA Kit
Human Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial , ECH1 ELISA Kit
This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.[provided by RefSeq]
Product Name:

Human Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial , ECH1 ELISA Kit

Product Type: ELISA Kit
Code: CSB-EL007378HU
Alias: HPXEL, delta3,5-delta2,4-dienoyl-CoA isomerase|dienoyl-CoA isomerase|peroxisomal enoyl-CoA hydratase 1|peroxisomal enoyl-coenzyme A hydratase-like protein
Size: 96T
Species: Human
Target Name: enoyl Coenzyme A hydratase 1, peroxisomal
Abbreviation: ECH1
Protein Biological Process 1: Biosynthesis/Metabolism
Protein Biological Process 2: Lipogenesis and lipometabolism
Protein Biological Process 3: Fatty acid metabolism
Detect Range: 15.6 pg/ml-1000 pg/ml
Sensitivity: 3.9 pg/ml
Assay Time: 1-5h
Sample Volume: 50-100ul
Detection wavelengt: 450 nm
Specificty: This assay has high sensitivity and excellent specificity for detection of Human ECH1. No significant cross-reactivity or interference between Human ECH1 and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): CV%<8% 
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10% 
Three samples of known concentration were tested in twenty assays to assess.
Sample collection and storage: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Assay procedure: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after tha...... 
Calculation of results: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be...... 


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