Human Eukaryotic translation initiation factor 4E-binding protein 1 , EIF4EBP1 ELISA Kit
Product Type:
ELISA Kit
Code:
CSB-EL007561HU
Alias:
4E-BP1, 4EBP1, BP-1, MGC4316, PHAS-I, eIF4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
Size:
96T
Species:
Human
Target Name:
eukaryotic translation initiation factor 4E binding protein 1
Abbreviation:
EIF4EBP1
Protein Biological Process 1:
Transcription/Transcription regulation
Protein Biological Process 3:
Translation regulation
Detect Range:
46.88 pg/ml-3000 pg/ml
Sensitivity:
11.72 pg/ml
Assay Time:
1-5h
Sample Volume:
50-100ul
Detection wavelengt:
450 nm
Specificty:
This assay has high sensitivity and excellent specificity for detection of Human EIF4EBP1. No significant cross-reactivity or interference between Human EIF4EBP1 and analogues was observed.
Precision:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Sample collection and storage:
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Assay procedure:
Bring all reagents and samples to room temperature before use. Centrifuge the sample again after tha......
Calculation of results:
Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be......