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Human Eukaryotic translation initiation factor 4E-binding protein 1 , EIF4EBP1 ELISA Kit
Human Eukaryotic translation initiation factor 4E-binding protein 1 , EIF4EBP1 ELISA Kit
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Product Name:

Human Eukaryotic translation initiation factor 4E-binding protein 1 , EIF4EBP1 ELISA Kit

Product Type: ELISA Kit
Code: CSB-EL007561HU
Alias: 4E-BP1, 4EBP1, BP-1, MGC4316, PHAS-I, eIF4E-binding protein 1|phosphorylated heat- and acid-stable protein regulated by insulin 1
Size: 96T
Species: Human
Target Name: eukaryotic translation initiation factor 4E binding protein 1
Abbreviation: EIF4EBP1
Protein Biological Process 1: Transcription/Transcription regulation
Protein Biological Process 3: Translation regulation
Detect Range: 46.88 pg/ml-3000 pg/ml
Sensitivity: 11.72 pg/ml
Assay Time: 1-5h
Sample Volume: 50-100ul
Detection wavelengt: 450 nm
Specificty: This assay has high sensitivity and excellent specificity for detection of Human EIF4EBP1. No significant cross-reactivity or interference between Human EIF4EBP1 and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): CV%<8% 
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10% 
Three samples of known concentration were tested in twenty assays to assess.
Sample collection and storage: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Assay procedure: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after tha...... 
Calculation of results: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be...... 


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