Home   |   Product Search   |   Site Map   |   Checkout   |   Track Your Order
Search by Keyword

Search by Keyword

Human Glutamate--cysteine ligase catalytic subunit , GCLC ELISA Kit
Human Glutamate--cysteine ligase catalytic subunit , GCLC ELISA Kit
Click to enlarge image(s)
Product Name:

Human Glutamate--cysteine ligase catalytic subunit , GCLC ELISA Kit

Product Type: ELISA Kit
Code: CSB-EL009321HU
Alias: GCL, GCS, GLCL, GLCLC, gamma-glutamylcysteine synthetase
Size: 96T
Species: Human
Target Name: glutamate-cysteine ligase, catalytic subunit
Abbreviation: GCLC
Protein Biological Process 1: Biosynthesis/Metabolism
Protein Biological Process 2: Amino-acid biosynthesis and metabolism
Protein Biological Process 3: Glutathione biosynthesis
Detect Range: 0.156 ng/ml-10 ng/ml
Sensitivity: 0.039 ng/ml
Assay Time: 1-5h
Sample Volume: 50-100ul
Detection wavelengt: 450 nm
Specificty: This assay has high sensitivity and excellent specificity for detection of Human GCLC. No significant cross-reactivity or interference between Human GCLC and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): CV%<8% 
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10% 
Three samples of known concentration were tested in twenty assays to assess.
Sample collection and storage: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Assay procedure: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after tha...... 
Calculation of results: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. 
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. 
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the GCLC concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. 
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.  


Copyright © egfie Herndon, VA
customer@egfie.com