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Plasmid Maxiprep Purification Kit
Plasmid Maxiprep Purification Kit
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Plasmid Maxiprep Purification Kit provides a unique Maxi-column and a high efficiency lysis buffer system to yield up to 800 μg high purity plasmid DNA from 100 to 400 ml bacterial cultures. The system containsendotoxin-removal reagent to eliminate eondotoxins. The high-purity plasmid DNA eluted using Plasmid Maxi Purification Kit is suitable for performing transfection experiments.

Features

  • Easy and Fast: Centrifugation and wash can be done in 50 ml centrifuge tube.
  • Safe: Does not require phenol/chloroform or CsCl/ ethidium bromide gradient centrifugation.
  • High Purity: Unique endotoxin removal formula, plasmid DNA purified can be used to perform sequencing, transfection or other experiments.
  • High Yield:  About 200~800 µg plasmid DNA can be purified from each reaction,  which is higher than the usual ion exchange method used by other suppliersbrands.


Maxi-Spin cloumn & 50 ml colection tube


Experimental Data

Test 1

Plasmid Maxiprep Kit yield and stability test


Purification of 4.6 kb plasmid DNA from 120 ml of E. coil using Plasmid Maxiprep Purification Kit.
The plasmid DNA was eluted with 1.5 ml of Elution buffer and analyzed using 0.8% agarose gel electrophoresis.
No. Elution vol. (μl) Final conc. (ng/μl) 260/280 260/230 Total yeild (μg) Average yeild (μg)
1 1270 800 1.89 2.22 1016 932.08
2 1190 760 1.92 2.28 904.4
3 1150 700 1.91 2.32 850
4 1500 620 1.86 2.26 930
5 1500 640 1.87 2.22 960


Test 2


Western blot analysis of transfection of purified pAkt plasmid using GeneMark Maxiprep Purification Kit or related kits from different suppliers.
Lane 1: BHK-21 cell lysate only.
Lane 2: BHK-21 cell lysate transfected with vector only.
Lane 3: BHK-21 cell lysate transfected with pAkt plasmid from supplier Q.
Lane 4: BHK-21 cell lysate transfected with pAkt plasmid from GeneMark.
General Procedure


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