The Blood Genomic DNA Purification Kit provides a rapid, simple and effective approach to isolate genomic DNA from various animal blood samples. The kit provides sufficient reagents to treat sample volume from 200 μl to 1.5 ml whole blood wiht three protocols. The process is based on a spin column format; the procedure involves cell lysis with proteinase K digestion, nucleic acids absorption and DNA elution. Typical yield ranges from 3~50 μg, depending on sample volume and species. DNA purified using this kit is suitable for various applications, including PCR, restriction enzyme digestion, cloning, dot blot analysis, etc.
Suitable for blood samples, provides three different protocols for different sample sizes ( 200 μl~1.5 ml blood) for optimized purification yield.
High yield: 3~50 μg.
High Purity: DNA purified is ready to use for subsequent applications such as cloning, PCR amplification, enzyme digestion, hybridization and sequencing.
Safe: No phenol/chloroform, the whole process can be done within 30 minutes.
Purification of rabbit whole blood genomic DNA using Blood Genomic DNA Purification Kit
1% purified DNA were loaded on 1% agarose gel.
Lane 1: 200 µl whole blood by protocol A in the kit
Lane 2: 500 µl whole blood by protocol B in the kit
Lane 3: 1.5 ml whole blood by protocol C in the kit