PCR clean-up kit

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The PCR Clean-Up Kit is designed to purify DNA fragments from PCR products or other enzymatic reaction (eg. Enzyme digestion, DNA ligation, probe labeling) based on our specific buffer system and high-capacity column. After the easy bind-wash-elute procedure, excess primers and salts are removed from PCR products or enzymatic reaction, and DNA fragments larger than 70 bp can be purified using this kit.

Features

Easy to handle: Spin Column format.
High efficiency: The recovery rate of PCR/DNA Clean-Up can range from 80~95%.
High purity: Efficiently removes primer or dimer. Purified DNA can be further used for enzyme digestion, labeling, ligation, hybridization, automated or manual sequencing and T-vector cloning.
Fast: The procedure can be completed within 15 minutes.

Experimental Data

Lane 1: Unpurified PCR product
Lane 2: Purified using GeneMark PCR Clean-Up Kit
Lane 3: Purified using related kit from supplier A
Lane 4: Purifiedusing related kit from supplier B

General Procedure

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