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Micro spin filter columns for IP, Co-IP, Immunodepletion, and affinity chromatography (column only), 50 preps

Description:

Micro Spin Filter provides an easy and efficient column method for Immunoprecipitation (IP), Co-IP, and Immunodepletion.

All the procedures involve 1) incubation of an antibody with a sample that contains the protein antigen of interest, 2)

capturing the antibody-antigen complex to immobilized Protein A or Protein G agarose beads, 3) washing to remove unbound

components of the sample, and 4) separation of antigen and antibody from beads.

Advantage of using spin filter column

 Traditional method: The entire procedure is performed in a microcentrifuge tube, which requires a) careful removal of

solution from agarose resin after pelleted by centrifugation and b) eluting IP-complex by boiling the resin in denaturing

sample loading buffer for SDS-PAGE analysis, which will denature and separate antibody into heavy and light subunits.

 Column method: Solution can be easily separated from the beads during the wash steps and the antibody-antigen complex

can be eluted by low pH buffer and neutralized for subsequent analysis instead of being denatured or cleaved into separate

subunits. Furthermore, the resin often can be reused to save the cost.

Highlights:
      Efficiently and effectively remove contaminating proteins from beads

   No resin loss, high recovery of antigen and co-precipitated proteins

   Low pH elution provides milder and less denaturing recovery of the antibody-antigen complex

   Fast and Easy with Spin protocol  

   Cost efficient by reusing affinity resin

Filter Disc does not bind to DNA/RNA or Proteins

Applications:

  Immunoprecipitation (IP)   Co-immunoprecipitation (co-IP)   Immunodepletion

  Other small scale affinity purification (affinity chromatography) such as Ni-resin, GST resin etc.


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