EvaGreen qPCR Mastermix is designed specifically for quantitative real-time analysis of miRNA samples. The components of EvaGreen qPCR Mastermix have been developed for superb performance in sensitivity, signal-to-noise ratio, and complete
elimination of primer dimers. The chemically modified Hotstart Taq polymerase included in our Mastermix significantly reduces non-specific PCR amplification observed with regular Taq polymerase.
Upon arrival, EvaGreen qPCR Mastermix should be stored at -20ºC and protected from light. After each experiment, the leftover thawed mix can be stored at 4ºC if it is to be used within the next 3 months.
Primer concentration should not be high. 100nM to 300nM each
- primer usually gives best results
- Melting curve is useful in distinguishing nonspecific amplification products and primer dimers from desired product. It's recommended to run melting curve following amplification cycles.
- To do replicates
- To reduce pipetting error: preparing amplicon specific master mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range are very effective to get tight Ct.
This product is distributed for laboratory research only.
Caution: Not for diagnostic use.