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IL-12 HUMAN

Synonyms:
NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.

Introduction:
IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity.

Description:
Interleukin-12 Human Recombinant produced in CHO is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.
The IL-12 is purified by proprietary chromatographic techniques.

Source:
CHO (Chinese Hamster Ovarian Cells)

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein (1mg/ml) was lyophilized with PBS pH-7.4 and 10% sucrose.

Solubility:
It is recommended to reconstitute the lyophilized Interleukin-12 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability:
Lyophilized Interleukin12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL12 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity:
Greater than 95.5% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
The sequence of the first five N-terminal amino acids was determined and found to be Arg-Asn-Leu-Pro-Val at the p35 subunit and Ile-Trp-Glu-Leu-Lys at the p40 subunit.

Biological Activity:
The ED50 was determined by the stimulation of IFN-gamma production by NK-cells co-stimulated with IL-18 and was found to be < 1ng/ml, corresponding to a specific activity of >1 x 106.

Usage:
ProSpec’s products are furnished for LABORATORY RESEARCH USEONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Size :
10ug


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