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LEPTIN BD HUMAN

Synonyms:
OB Protein, Obesity Protein, OBS, Obesity factor.

Description:
Leptin Binding Domain Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 208 amino acids and having a molecular mass of 24.5 kDa.
The Leptin Binding Domain is purified by proprietary chromatographic techniques.

Source:
Escherichia Coli.

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation:
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

Solubility:
The lyophilized Leptin Binding Domain is very soluble in water and most aqueous buffers below and above the isoelectric point.

Stability:
Lyophilized Leptin Binding Domain although stable at room temperature, should be stored desiccated below 0°C. Reconstituted Leptin is best stored refrigerated at 4°C.
Please prevent freeze-thaw cycles.

Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence:
The sequence of the first four N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val

Biological Activity:
Biological Activity is evidenced by high affinity binding of mammalian leptins at 1:1 molar ratio.

Protein content:
Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 2.45 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of Leptin Binding Domain as a Reference Standard.

Usage:
ProSpec’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Size:
50ug


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