This recombinant human adenovirus type 5 expresses EYFP-Actin which encodes a fusion of the enhanced yellow fluorescent protein (EYFP) and human cytoplasmic beta-actin. EYFP is an enhanced yellow-green variant of the Aequorea victoria green fluorescent protein (GFP). The EYFP gene contains the four amino acid substitutions previously published as GFP-10C: Ser-65 to Gly; Val-68 to Leu; Ser-72 to Ala; and Thr-203 to Tyr. The fluorescence excitation maximum of EYFP is 513 nm; the emission spectrum has a peak at 527 nm. This control adenovirus will eventually infect all mammalian cells and will be used to visualize the network of microfilaments.
Product Category: human Adenovirus Type5 (dE1/E3). Ad-EYFP-Actin or Ad-CMV-EYFP-Actin