• Unit Size: 1 x 0.2 ml

Product Category: human Adenovirus Type5 (dE1/E3), mCherry-PA-Rac1 adenovirus for over-expression of mCherry-PA-Rac1 under CMV promoter; Ad-CMV-mCherry-PA-Rac1

Description: Rac1 is a key GTPase regulating actin cytoskeletal dynamics in metazoan cells. Rac1 mutants were fused to the photoreactive LOV (light oxygen voltage) domain from phototropin, sterically blocking Rac1 interactions until irradiation unwound a helix linking LOV to Rac1. Photoactivatable Rac1 (PA-Rac1) could be reversibly and repeatedly activated using 458- or 473-nm light to generate precisely localized cell protrusions and ruffling. Localized Rac activation or inactivation was sufficient to produce cell motility and control the direction of cell movement. Myosin was involved in Rac control of directionality but not in Rac-induced protrusion, whereas PAK was required for Rac-induced protrusion. PA-Rac1 was used to elucidate Rac regulation of RhoA in cell motility. Rac and Rho coordinate cytoskeletal behaviours with seconds and submicrometre precision. A mCherry reporter was then cloned at N-terminal of PA-Rac1 to generate mCherry-PA-Rac1 construct which was then packaged to adenovirus.  Ad-CMV-mCherry-PA-Rac1 can be used to infect most of mammalian cell types for time-lapse visualization of Rac1 activation via photoactivation.  

Titer: 1x1010~1x1011 PFU/ml 

Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol

User Manual: 

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