EGFIE LLC
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Lentiviral vector is a powerful tool for delivering target genes into almost all types of dividing and non-dividing mammalian cells in vitro and in vivo. However, production of recombinant lentiviral vectors (>107 transducing unit (TU)/ml) is required.
We manufacture both Custom and Ready-to-use lentiviral vectors.
Our standardized and optimized custom lentiviral vector production and strict quality control systems enables us to generate high titer of functional recombinant lentiviral vectors for our customers, Our titration procedures determine the quantity of functional lentiviral vectors rather than physical lentiviral particles.
| Cat. No | Service Description | Vector Titer (TU/ml) | Volume | Turnaround Time (weeks) | Price (construct generation not included) |
| CLVP-106-010ML | Custom Crude Lentiviral Vector Production | N/A | 10ml | 2-3 | #350.00 |
| CLVP-107-001ML | Custom Low-Titer of Lentiviral Vector Production | >107 | 1ml | 2-3 | $1190.00 |
| CLVP-108-200UL | Custom Medium-Titer fo Lentiviral Vector Production | >108 | 200μl | 2-3 | $1590.00 |
| CLVP-109-200UL | Custom High-Titer of Lentiviral Vector Production | >109 | 200μl | 2-3 | $2190.00 |
| CLVP-110-020UL | Custom Ultra-High-Titer of Lentiviral Vector Production | >1010 | 20μl | 2-3 | $2490.00 |
| CLVP-Quota | Custom-Titer and Scale of Lentiviral Vector Production | Custom | Custom | 2-3 | Quote |
Notes: # One titer unit of EGFIE is roughly equivalent to more than 5 units of IFU/ml or TU/ml from our competitor.
## Customer should provide enough amount of sterile, endotoxin-free ready-for-transfection transfer vector DNA for virus production.
### Customer should provide enough amount of sterile, endotoxin-free ready-for-transfection transfer vector DNA for virus production.
#### Titer is measured in clone formation unit (CFU) when antibiotics resistant gene is available in construct; titer in transducing units when fluorescence marker is available is construct; p24 ELISA or other assays may be used if none of them is feasible.
How custom lentiviral production service proceeds:
If lentiviral construct is ready, please provide us:
1. The construct may or sequence.
2. 1-5μg of your lentiviral construct plasmid DNA or bacterial culture.
We will prepare Endotoxin-free plasmid and transfect our packaging cell line for your lentivirus production. Harvest lentivirus particle followed by concentration and titration.
You will receive >200μl of lentiviral vectors in 25μl aliquots as indicated in the Table above.
If you need us to generate lentiviral vector for your target gene, please:
1. Choose lentiviral vector format from our vector collection and specify the gene of interest.
2. Please send us 1-5μg plasmid or bacterial culture for your gen if it is available, otherwise send us cDNA dequence.
We will subclone the gene of interest or shRNA into chosen lentiviral vector and confirm by dequencing, prepare high quality plasmid DNA and transfect packaging cell line, harvest lentivirus particle followed by concentration and titration.
You will receive >200μl of lentiviral vectors in 25μl aliquots as indicated in the Table above.
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Note: All prices in US Dollars
customer@egfie.com