EGFIE LLC
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Based on our unique and proprietary “VIPs” and polymer synthesis technologies, currently we are developing and manufacturing three categories of DNA/siRNA transfection reagents, which show distinct transfection characteristics compared with the leading products in the market. What's more important is that the transfection reagents we are manufacturing are very affordable and will save your precious research budget.
Transfection Reagent Selection Chart.
| Reagents | Features | Transfection Type | Toxicity |
| DNAfect | - Traditional recipe with new formulation - Most affordable and efficient - Best for large scale virus, antibody and protein production |
DNA siRNA DNA/siRNA |
++++ |
| OKDNAfect | - Low toxicity for very sensitive cells - Maximum cell viability |
DNA siRNA DNA/siRNA |
++ |
| SupDNAfect | - 100% DNA condensing groups released - Efficient for commonly used cell types |
DNA siRNA DNA/siRNA |
+++ |
| SupDNAfect Plus | - Enhanced conjugate for maximal efficiency - Extremely good for very long DNAs |
DNA siRNA DNA/siRNA |
++ |
| LenDNAfect | - Enhanced conjugate with liposome for maximal efficiency - Best for lentivirus, antibody and protein production |
DNA siRNA DNA/siRNA |
+++ |
| AllDNAfect | - Fluorinated cationic lipids - Best transfection efficiency with least toxicity |
DNA siRNA DNA/siRNA |
+ |
| ExcelDNAfect | - Biodegradable polycationic polymer - Excellent efficiency for common used mammalian cells |
DNA siRNA DNA/siRNA |
+++ |
Description
DNAfect Tranfection Reagent is a pre-optimized and refined version of traditional calcium phosphate transfection reagent. With a new chemistry, DNAfect Reagent contains much lower concentration of calcium, leading to much lower cytotoxicity without sacrificing transfection efficiency. In addition, DNAfect Reagent is an effective method for the production of long-term stable and transient transfectants on most adherent cell lines. compared with liposome and polymer based transfection reagents, DNAfect Reagent shows distinguished features and much higher efficiency on HepG2, HEK293, CHO, Hela, COS, 3T3, LNcap, C6, PC12 and primary cultured cells, etc. DNAfect Reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
Application
High-efficiency stable or transient transfection of eukaryotic cells.
Feature
- High efficient: Components optimized for high transfection efficiencies
- Simple procedures: Total one tube reaction
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Comparisons of Transfection Efficiency of DNAfect Reagent with the Leading Brand Name Product
A comparison study showing transfection efficiency of DNAfect Reagent vs. a leading product, Lipofectamine 2000 on HEK293T cells. HEK293T cells were transfected with GFP tagged DNA (total 14 kb) by DNAfect (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection
Technical Information & Datasheet
- A General Protocol for Transfecting Mammalian Cells
- A Protocol for Lentivirus Production
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