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SupDNAfect Plus
In Vitro DNA Transfection Reagents:

Based on our unique and proprietary “VIPs” and polymer synthesis technologies, currently we are developing and manufacturing three categories of DNA/siRNA transfection reagents, which show distinct transfection characteristics compared with the leading products in the market. What's more important is that the transfection reagents we are manufacturing are very affordable and will save your precious research budget.

Transfection Reagent Selection Chart.

Reagents Features Transfection Type Toxicity
DNAfect - Traditional recipe with new formulation
- Most affordable and efficient
- Best for large scale virus, antibody and protein production
 DNA
 siRNA
 DNA/siRNA
++++
OKDNAfect - Low toxicity for very sensitive cells
- Maximum cell viability
 DNA
 siRNA
 DNA/siRNA
++
SupDNAfect - 100% DNA condensing groups released
- Efficient for commonly used cell types
 DNA 
 siRNA
 DNA/siRNA
+++
SupDNAfect
Plus 
- Enhanced conjugate for maximal efficiency
- Extremely good for very long DNAs
 DNA 
 siRNA
 DNA/siRNA
++
LenDNAfect - Enhanced conjugate with liposome for maximal efficient- Best for lentivirus, antibody and protein production  DNA
 siRNA
 DNA/siRNA
+++
AllDNAfect - Fluorinated cationic lipids
- Best transfection efficiency with least toxicity
 DNA
 siRNA
 DNA/siRNA
+
ExcelDNAfect - Biodegradable polycationic polymer
- Excellent efficiency for common used mammalian cells
 DNA
 siRNA
 DNA/siRNA
+++
Description
SupDNAfect Plus DNA ranfection Reagent is enhanced version of GenJet™ DNA In Vitro Transfection Reagent. SupDNAfect Plus is formulated by addition of a booster peptide, giving rise to up to 20 times higher efficiency on variety of mammalian cells in comparison of its previous version. SupDNAfect Plus was shown to efficiently deliver genes to various established cell lines as well as primary cells including HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines with less toxicity. SupDNAfect Plus reagent, 1.0 ml, is sufficient for 667 transfections in 24 well plates or 333 transfections in 6 well plates.

Features compared with old version SupDNAfect Transfection Reagent
- Cell-dependent 3~20 times higher efficiency
- Top choice for hard-to-transfect cells 
- Equally good for very long DNAs (up to 46 kb) 
- Efficiency boosted in the presence of serum and antibiotics for most of cell types
- Exceptional high levels of recombinant protein production

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Comparisons of Transfection Efficiency of SupDNAfect Transfection Reagent with the Leading Brand Name Products

SupDNAfect Plus is top choice for hard-to-transfect cells. Efficiency comparison of SupDNAfect Plus In Vitro DNA Transfection Reagent (left panel) with Lipofectamine 2000 (L2K, right panel) on primary cultured rat aortic smooth muscle cells. A nuclear targeted GFP DNA was transfected with SupDNAfect Plus and L2K respectively per manufacturer's protocols. The efficiency was checked 48 hours post transfection. The above pictures were kindly provided by Dr. Nickolai Dulin of Section of Pulmonary and Critical Care, University of Chicago


Left Panel: Comparison of SupDNAfect Plus In Vitro DNA Transfection Reagent with leading products including Lipofectamine 2000 (L2K) and Lipofectamine LTX (LTX) on lentivirus production on 293T cells. The data were provided by Dr. Miguel Toscano of Temple University as courtesy. 
Right panel: Comparison of SupDNAfect Plus In Vitro DNA Transfection Reagent with tranfection reagents from other vendors inluding Lipofectamine 2000 (L2K) and FuGene 6 on Hela cells. HeLa cells were transfected with plasmid DNA encoding GFP. Transfections were performed according to manufacturers recommendations and analysed by flow cytometry.



Exceptional high titers of lentivirus generated from 293T cells. Three DNAs co-transfected with SupDNAfect Plus In Vitro DNA Transfection Reagent (left panel) in comparison of Lipofectamine LTX (right panel). 1.0 and 10 microliters virus supernatant from 293T cells were used to counter-infected 293F cells which was then passed FACS. The numbers at the upper right corner indicate the percentage of transduced cells. The titers of lentivirus generated with SupDNAfect Plus and Lipofectamine LTX were quantified to be 2.1x10and 9.3x106 tu/ml respectively. The above data were provided by Dr. Miguel Toscano of Temple University as courtesy. 

Technical Information & Datasheet
GenJet™ Plus Data Sheet & General Protocol 
GenJet™ Plus In Vitro DNA Transfection for Lentivirus Production
GenJet™ Plus In Vitro DNA Transfection for rAAV Production
Transfection of Sf9 Insect Cells with GenJet™

Related Product: GenJet™ In Vitro DNA Transfection reagent

To request a free trial sample, please email us at customer@egfie.com

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