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ExcelDNAfect
In Vitro DNA Transfection Reagents:

Based on our unique and proprietary “VIPs” and polymer synthesis technologies, currently we are developing and manufacturing three categories of DNA/siRNA transfection reagents, which show distinct transfection characteristics compared with the leading products in the market. What's more important is that the transfection reagents we are manufacturing are very affordable and will save your precious research budget.

Transfection Reagent Selection Chart.

Reagents Features Transfection Type Toxicity
DNAfect - Traditional recipe with new formulation
- Most affordable and efficient
- Best for large scale virus, antibody and protein production
 DNA
 siRNA
 DNA/siRNA
++++
OKDNAfect - Low toxicity for very sensitive cells
- Maximum cell viability
 DNA
 siRNA
 DNA/siRNA
++
SupDNAfect - 100% DNA condensing groups released
- Efficient for commonly used cell types
 DNA 
 siRNA
 DNA/siRNA
+++
SupDNAfect
Plus
- Enhanced conjugate for maximal efficiency
- Extremely good for very long DNAs
 DNA 
 siRNA
 DNA/siRNA
++
LenDNAfect - Enhanced conjugate with liposome for maximal efficiency
- Best for lentivirus, antibody and protein production
 DNA
 siRNA
 DNA/siRNA
+++
AllDNAfect - Fluorinated cationic lipids
- Best transfection efficiency with least toxicity
 DNA
 siRNA
 DNA/siRNA
+
ExcelDNAfect - Biodegradable polycationic polymer
- Excellent efficiency for common used mammalian cells
 DNA
 siRNA
 DNA/siRNA
+++
Description
Based on our proprietary polymer synthesis technology, ExcelDNAfect Transfection Reagent is formulated as a biodegradable polymer based DNA transfection reagent that ensures effective and reproducible transfection on HEK293, COS-7, NIH-3T3, HeLa, CHO and a broad ranges of hard-to-transfect mammalian cells. ExcelDNAfect reagent is able to immobilize DNA migration during electrophoresis at very low concentration and form transfection complex within 5 minutes at RT. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis (Figure 1), leading to much less cytotoxicity. PolyJet™ reagent, 1.0 ml, is sufficient for ~667 transfections in 24 well plates or ~333 transfections in 6 well plates, providing a very affordable alternative to the leading products for transfecting a variety of commonly used and hard-to-transfect mammalian cells.


Figure 1. A Cartoon Showing Biodegradation of ExcelDNAfect Transfection Reagent After Endocytosis of Transfection Complex

Features
- Bio-degradable after endocytosis
- Exceptional high titers of virus production
- Equally good for very long DNAs (>89 kb)
- Equally good for both single DNA transfection and multi DNA co-transfection
- High levels of recombinant protein production
- Simple & robust transfection procedure
- Very affordable

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

Broad Transfection Spectrum for Mammalian Cell Types
Cell Lines Efficiency (% GFP) Cell Lines Efficiency (% GFP)
McArdle 7777
Hep3D
SHEP
3T3-442A
COS-7
CV-1
D 407
DHD Pro.b 
3LL
B16-F10
BAEC
BHK-21
Ca Ski
CaCo2
CHO
HCS-2/8
HEK-293
HeLa
HLMEC
H-MVEC
Huh-7D12 
ATT20
SK-N-SH
C2C12
HepG2
65-70%
67-76%
68-71%
35%
85-90%
60%
70%
70%
80%
85%
51%
80%
88%
60%
88%
61%
86%
88%
72%
59%
72%
46%
29%
46%
72%
SAOS-2
SN56
MC3T3-E1
Primary melanocyte
K562
L929
MCF-7
MDCK
Neuro2A
NIH 3T3
PC12
SH-SY5Y
SiHa
SKOV3
Huh-7
IGROV1
DF-1, Chicken Embryonic Cell
6CSFMEo
WEHI 231
A549
LNCap
Prim. mouse keratinocyte
Prim. human skin fibroblast
Prim. human pre-adipocyte
Prim. mouse embry. fibroblast
58%
81%
80%
35%
38%
59%
68%
68%
86%
76%
50%
25%
60%
65%
70%
35%
50%
71%
26%
75%
75
29%
50%
32%
30%

Examples Showing Transfection Efficiency of ExcelDNAfect Transfection Reagent on Commonly Used Cells
PolyJet_L2K_CHO
Transfection efficiency comparison of ExcelDNAfect vs. lipofectamine Plus on Chinese Hamster Ovary (CHO) cells. HA tagged beta-tubulin cDNA was delivered into CHO cells with PolyJet™ (left panel) and lipofectamine Plus (right panel) respectively. FITC conjugated antibody against HA tag was utilized to pick up HA-beta-tubulin (Green) while a DM1a antibody was used to detect endogenous alpha-tubulin followed by probing with rhodamine conjugated secondary antibody (Red). The above picture was provided by Dr. Shang Yin of University of Texas at Houston Medical School as courtesy

PolyJet_L2K_HEK293
A comparison showing transfection efficiency of ExcelDNAfect reagent vs. a leading product, Lipofectamine 2000 on HEK293FT cells.HEK-293FT cells were transfected with GFP vector (pEGFP-N3) by ExcelDNAfect (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection

PolyJet_L2K_HepG2
A comparison showing transfection efficiency of ExcelDNAfect reagent vs. a leading product, Lipofectamine 2000 on HepG2 cells.HepG2 cells were transfected with GFP vector (pEGFP-N3) by ExcelDNAfect (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection

PolyJet_Fugene_HD_MDCK
Transfection efficiency comparison of ExcelDNAfect vs. Fugene HD on MDCK cells. A plain GFP DNA was transduced into MDCK cells with PolyJet™ (left panel) and Fugene HD (right panel) reagents respectively per manufacturers' protocols. GFP and DAPI staining were visualized under fluorescence microscopy 48 hours post tansfection. The above comparison data and pictures were completed and provided by Dr. Ge Zhou of NYU Medical Center as courtesy

PolyJet_L2K_MDCK
A comparison showing transfection efficiency of ExcelDNAfect reagent vs. a leading product, Lipofectamine 2000 on MDCK cells. MDCK cells are notoriously hard to transfect. With proprietary "Shaved Cell Transfection" protocol, ExcelDNAfect (left panel) gives up to 70% GFP positive cells vs. Lipofectamine 2000 (right panel) around 5% efficiency. MDCK cells were transfected with GFP vector (pEGFP-N3) by PolyJet™ (left panel) and Lipofectamine 2000 (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 36 hours post transfection

PolyJet_Fugene_HD_LNCap
A comparison showing transfection efficiency of ExcelDNAfect reagent vs. a leading product, Fugene HD on LNCap cells. LNCap cells were transfected with GFP vector (pEGFP-N3) by PolyJet™ (left panel) and Fugene HD (right panel) respectively. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection

PolyJet_L2K_N2A
Neuro2A cells transfected with pEGFP-C1 plasmid using ExcelDNAfect Transfection Reagent. The Neuro2A cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging (left) and FITC imaging (right) 24 hours post-transfection

PolyJet_L2K_Primary_Fibroblast
Comparison of cytotoxicity of ExcelDNAfect DNA In Vitro Transfection Reagent with L2K™ on primary murine skin fibroblast. The primary murine fibroblast was incubated with the indicated transfection reagents/pEGFP-C1 (DNA) complexes above for 4 hours in serum-free DMEM High Glucose medium followed by replacement of complete serum-containing medium. The cells were visualized by Nikon Eclipse Fluorescence microscope with DIC phase imaging 24 hours post transfection

Data Sheet and Protocols
General Protocol for Transfecting Mammalian Cells 
A Short Protocol for Transfecting Mammalian Cells 
Advanced Protocol for Transfecting Hard-to-Transfect Mammalian Cells 
Protocol for Lentivirus Production 
Protocol for rAAV Production 

Testimonials:

PolyJet Transfection Reagent worked equally as well as lipofectamine 2000, with little evidence of cell death on 293, PC-3 and 22RV1 cells. I will defiantly consider switching over.
-----Tiffany Wallace, Ph.D., NCI / NIH

I only did side by side with the testing sample (PolyJet) and Lipo2000 with GFP transfection on COS-7 cells. The result was very good. PolyJet was even better than L2K.
------Feng Qiao, Ph.D., NEI / NIH

I tested the sample of PolyJet on my NIH-3T3 mouse fibroblasts this weekend. The results were much better than Lipofecatmine LTX. I'm attaching a powerpoint slide with my results (I did not quantify the % transfection efficiency, but the pictures get the point across). I found that the protocol for difficult-to-transfect cell lines worked better than the standard protocol.
-----Stephanie Murphy, Ph.D., Dartmouth College

I had chance to try your product finally. It was great success. I used HeLa cells and got 10% transfection efficiency (<0.1% for Lipofectamine). Thank you! I was wondering if I also try GenJet™ Plus DNA In Vitro Transfection Reagent? According to your website, the reagent works better than regular PolyJet.
-------Yumi Uetake, Ph.D., UMASS

I tested PolyJet and it looks great on MDCK. We placed order. Thank you!
-------Ge Zhou, Ph.D., NYU

We are happy to provide feedback. PolyJet worked very well for us in HepG2 cells, we got approximately 80% efficiency with pMAX GFP plasmid, by following the conditions in your suggested protocol. We ran a comparison with Lipofectamine, which only showed approximately 20-30% transfection efficiency. We are planning experiments and will be ordering more soon.
-------Emily Mcallister, PBRC


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