Description: 

Immunohistochemistry (IHC) is a method of detecting the presence of specific proteins in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. IHC is widely used in the diagnosis of abnormal cells and basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue. 

This product is based on a novel LSAB (labeled streptavidin-biotin) method. In this new method a single primary antibody is subsequently associated with multiple biotin molecules. Poly-HRP Streptavidin provides multiple active biomolecules on each polymer chain which increase the biotin binding capacity and amplify the peroxidase enzyme signal. 

Contents: 

1. RTU normal goat serum: 10ml. 

2. RTU peroxidase blocking solution: 10ml 

3. RTU biotinylated anti-rabbit secondary antibody: 10ml 

4. RTU streptavidin-HRP polymer: 10ml 

Note: RTU=Ready-to-use 

Storage 

Store at 2-8°C. 

 Protocol: 

1. Preparation of Slides 

A. Cell Lines 

 Grow cultured cells on sterile glass cover slips or slides overnight at 37 º C 

 Wash briefly with PBS 

 Fix as desired. Possible procedures include: 

a. 20 minutes with 10% formalin in PBS (keep wet) 

b. 10 minutes with ice cold methanol, allow to air dry 

c. 10 minutes with ice cold acetone, allow to air dry 

 Wash in PBS