Rat Osteocalcin/Bone gla protein , OT/BGP ELISA Kit
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Product Type: ELISA Kit
Code: CSB-E05129r
Alias: RP11-54H19.5, BGP, OC, PMF1, bone Gla protein|bone gamma-carboxyglutamate (gla) protein (osteocalcin)|gamma-carboxyglutamic acid-containing protein|osteocalcin
Size: 96T
Species: Rat
other species: Bovine Dog Goat Human Monkey Mouse Pig Rabbit
Target Name: bone gamma-carboxyglutamate (gla) protein
Abbreviation: BGLAP
Protein Biological Process 1: Biosynthesis/Metabolism
Protein Biological Process 3: Biomineralization
Detect Range: 0.312 pg/ml-20 pg/ml
Sensitivity: 0.078 pg/ml
Assay Time: 1-5h
Sample Volume: 50-100ul
Detection wavelengt: 450 nm
Specificty: This assay has high sensitivity and excellent specificity for detection of Rat BGLAP. No significant cross-reactivity or interference between Rat BGLAP and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): CV%<8% 
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10% 
Three samples of known concentration were tested in twenty assays to assess.
Sample collection and storage: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Assay procedure: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after tha...... 
Calculation of results: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be...... 

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