AC1, 3 ,5 -cyclic AMP synthetase|ATP pyrophosphate-lyase|Ca(2+)/calmodulin-activated adenylyl cyclase|adenyl cyclase|adenylate cyclase 1|adenylate cyclase type I
adenylyl cyclase 1(AC-1)
Protein Biological Process 1:
Protein Biological Process 2:
Adenosine synthesis and metabolism
Protein Biological Process 3:
31.25 pg/ml-2000 pg/ml
This assay has high sensitivity and excellent specificity for detection of Rat AC-1. No significant cross-reactivity or interference between Rat AC-1 and analogues was observed.
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Sample collection and storage:
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Bring all reagents and samples to room temperature before use. Centrifuge the sample again after tha......
Calculation of results:
Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be......