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Rat Steroid 17-alpha-hydroxylase/17,20 lyase , CYP17A1 ELISA Kit
Rat Steroid 17-alpha-hydroxylase/17,20 lyase , CYP17A1 ELISA Kit
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Product Name:

Rat Steroid 17-alpha-hydroxylase/17,20 lyase , CYP17A1 ELISA Kit

Product Type: ELISA Kit
Code: CSB-EL006392RA
Alias: RP11-753C18.1, CPT7, CYP17, P450C17, S17AH, cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia|cytochrome p450 XVIIA1|steroid 17-alpha-hydroxylase/17,20 lyase|steroi
Size: 96T
Species: Rat
other species: Human
Target Name: cytochrome P450, family 17, subfamily A, polypeptide 1
Abbreviation: CYP17A1
Protein Biological Process 1: Biosynthesis/Metabolism
Protein Biological Process 2: Lipogenesis and lipometabolism
Protein Biological Process 3: Steroidogenesis
Detect Range: 25 pg/ml-1600 pg/ml
Sensitivity: 6.25 pg/ml
Assay Time: 1-5h
Sample Volume: 50-100ul
Detection wavelengt: 450 nm
Specificty: This assay has high sensitivity and excellent specificity for detection of Rat CYP17A1. No significant cross-reactivity or interference between Rat CYP17A1 and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): CV%<8% 
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10% 
Three samples of known concentration were tested in twenty assays to assess.
Sample collection and storage: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Assay procedure: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after tha...... 
Calculation of results: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be...... 


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