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Mouse Glucokinase , GCK ELISA Kit
Mouse Glucokinase , GCK ELISA Kit
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Product Name:

Mouse Glucokinase , GCK ELISA Kit

Product Type: ELISA Kit
Code: CSB-EL009319MO
Alias: GK, GLK, HHF3, HK4, HKIV, HXKP, MODY2, ATP:D-hexose 6-phosphotransferase|glucokinase|hexokinase D, pancreatic isozyme
Size: 96T
Species: Mouse
other species: Human Rat
Target Name: glucokinase (hexokinase 4)
Abbreviation: GCK
Protein Biological Process 1: Biosynthesis/Metabolism
Protein Biological Process 2: glyconeogenesis and glycometabolism
Protein Biological Process 3: Glycolysis
Detect Range: 15.6 pg/ml-1000 pg/ml
Sensitivity: 3.9 pg/ml
Assay Time: 1-5h
Sample Volume: 50-100ul
Detection wavelengt: 450 nm
Specificty: This assay has high sensitivity and excellent specificity for detection of Mouse GCK. No significant cross-reactivity or interference between Mouse GCK and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): CV%<8% 
Three samples of known concentration were tested twenty times on one plate to assess. 
Inter-assay Precision (Precision between assays): CV%<10% 
Three samples of known concentration were tested in twenty assays to assess.
Sample collection and storage: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Assay procedure: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after tha...... 
Calculation of results: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be...... 


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