Real-time size fractionation and recovery of nucleic acids

runVIEW is an innovative new system which can be used to maximise the efficiency of DNA recovery from EtBr and SYBR stained gels by minimising the number of steps involved in postelectrophoretic purification. runVIEW consists of a multiSUB™ MSCHOICE system with bluVIEW lid, containing an orange spectral emission filter within its viewing pane, and a base unit with integrated power supply and blue LED gel illuminator.

Simple DNA recovery

Using runVIEW is simple: first, cast a gel featuring two rows of wells with one matching pair of the preparatory combs supplied, before transferring the blue-light transparent gel tray to the MSCHOICE tank located on the base unit. Add sufficient buffer just to cover the gel, and remove the combs to load the DNA samples into the upper row of wells (‘Loading’ tier). Replace the lid to connect the MSCHOICE tank to the integrated power supply before applying the voltage and switching on the blue LED gel illuminator. Watch through the bluVIEW lid’s viewing pane as the samples migrate in real-time to the second row of wells (‘extraction’ tier). Once the DNA bands of  nterest enter the ‘extraction’ tier, simply stop the power supply, remove the lid and harvest the DNA by pipette.

Track DNA without harmful UV

Blue light is safe and has none of the detrimental effects of UV transillumination, including mutagenesis which can compromise cloning efficiency, while runVIEW’s capacity to provide real-time visualisation of electrophoresis enables DNA to be tracked as it migrates through the gel. This allows the user to judge precisely when the band of interest is ready for extraction.

Save on time-consuming gel elution techniques

Running DNA into a well containing buffer effectively purifies the DNA of agarose, eliminating the need for time-consuming gel excision and purification techniques that also result in sample loss. Once harvested, the DNA needs only to be cleaned by a straightforward ethanol precipitation when it is ready for further digestion or ligation.

No expensive commercial gels

runVIEW works with standard EtBr, SYBR Green and SYBR Safe gels cast within the 15x7, 15x10 or 15x15cm MSCHOICE gel trays, and therefore does not require expensive precast gels and accessories.

A self-contained system

The base unit, which houses the in-built power supply and blue LED gel illuminator, is compact, dual-voltage and portable, and allows electrophoresis, gel visualisation and extraction to be performed at the bench, without the inconvenience of having to transport gels to a darkroom elsewhere within the laboratory.

Other benefits offered by runVIEW include:

 
• Power supply integrated within the base unit – adjustable in precise 1V or 1mA increments
to a maximum 150V or 300mA constant voltage or current output; timer function to 999
minutes for extended runs.

• Specialist combs for specialist applications - double-sided 1mm preparatory combs
(1- / 2-sample and 4- / 16-sample standard) included for nucleic acid recovery, plus four
multichannel compatible 20- / 28-sample combs for rapid screening of nucleic acids from
96-well thermal cycler blocks and microtitre plates.

• Get real-time - existing MSCHOICE users may customise their system by purchasing the
base station and bluVIEW lid.

runVIEW Specifications: