Description

The ExcelDye 6× DNA Loading Dye (Green) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 800 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Contents

Component                                                           Volume

ExcelDye 6× DNA Loading Dye, Green                2ml × 2

Composition

0.03% Xylene cyanol FF 
0.15% Orange G 
10 mM Tris-HCl (pH 8.0) 
60% glycerol

Storage

  4°C  ≥ 12 months

-20°C ≥ 36 months