• Easy to operate:Spin Column format.
• High yield:Can prepare 10~40 μg plasmid DNA from 1~5 ml culture.
• High Purity:High purity plasmid DNA that can be used directly for automatic sequencing, in vitrotranscription, PCR and enzyme digestion or any other subsequent applications.
• Fast:The whole process can be done within 10～15 minutes.

Supplier	A260/A280	Yield	Average
Supplier Q	1.86	7.1 μg	7.33 μg
	1.79	7.4 μg
	1.83	7.5 μg
Supplier Ga	1.94	6.3 μg	6.0 μg
	1.94	6.3 μg
	1.96	5.4 μg
GeneMark	1.94	11.3 μg	11.17 μg
	1.97	10.8 μg
	1.94	11.4 μg

Figure 1. Gel electrophoresis analysis of plasmid purified using plasmid purification kit from different suppliers.

Lane M: 100 bp DNA ladder

Lane Q: Supplier Q

Lane Ga: Domestic supplier Ga

Lane GeneMark: GeneMark

Figure 2. Sequencing result of plasmid DNA purified using GeneMark Plasmid DNA Purification Kit.

Detection signals are clear and easy to define, indicating plasmid DNA purified using GeneMark Plasmid DNA Purification Kit is of high purity, and the buffer system does not affect subsequent applications.

Figure 3. Purification of pUC18 using 15 randomly chosen spin columns to test the stability of the kit.

Lane 1~15: pUC18 purified using 15 spin columns.

Lane M: Gen-kb DNA Ladder