Plasmid Miniprep Purification Kit II is designed especially for endonuclease-rich bacteria strains such as BL21(DE3) and HB101. It contains endonuclease-removal reagent which eliminates endonuclease to protect DNA from degradation. Using the unique silica column and a high-efficiency lysis buffer system, this kit can yield up to 40 μg high purity plasmid DNA from 1 to 5 ml bacterial cultures, without needing to precipitate, concentrate, or desalt.
Easy to operate: Spin Column format
High Yield: Can purify 40 μgultra pure plasmid DNA from 1~5 ml bacterialculture.
High Purity: High purity plasmid DNA that can be used directly forautomatic sequencing, in vitro transcription, PCRand enzyme digestion or any other related experiments.
Fast: The whole process can be done within 10~15 minutes.
Exclusive formula: Effective removal of endonuclease, especially effective for EndA+ bacteria, L21(DE3), HB101 and JM109.
Figure 1. 2 ml Mini-preparation of pET29 recombinant in BL21 (DE3) using kits from three different suppliers.
After elution, add Hind III reaction buffer to final 1X concentration, incubation at 37℃ for 2 hours.
M: Gen-kb ladder
1: Purified by GeneMark Plasmid Miniprep Purification Kit II