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Plasmid midiprep purification kit
Plasmid midiprep purification kit
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Plasmid Midiprep Purification Kit contains a unique Midi-spin column and a high-efficiency lysis buffer system to yield 100 to 300 μg of high purity plasmid DNA from 20 to 100 ml bacterial cultures. The buffer system removes both endotoxinand endonuclease, therefore, high-purity plasmid DNA purified using this kit is suitable to perform subsequent applications such as PCR, restriction digestion, cloning, automated DNA sequencing, southern blot, in vitro transcription assay and transfection.
Features
  • Easy to operate: Spin Column format, similar to miniprep.
  • High Yield: Each reaction is capable of purifying 100~300 µg plasmid DNA from  20~100 ml bacterial suspension.
  • High Purity: Plasmid DNA purified with removal of endotoxins, and can perform transfectionand automatic sequencing.
  • Fast: The whole process can be done within an hour.
  •             

Experimental Data


Figure 1. Gel electrophoresis analysis of 6.3 kb plasmid DNA purified from 40 ml culture using
GeneMark Plasmid Midiprep Purification Kit (DP01MD) and with related kit from supplier Q.
M: GenKB DNA Ladder | Q: Using related kit from Supplier Q | GM: GeneMark Plasmid Midiprep.

Supplier Final concentration A260/A280 Yield Average
Supplier Q 606 ng/µl 1.9 181.8 µg 184.6 µg
624 ng/µl 1.89 187.2 µg
616 ng/µl 1.87 184.8 µg

GeneMark
708 ng/μl 1.91 212.4 μg 211.1 μg
910 ng/μl 1.91 273.0 μg
692 ng/μl 1.91 207.6 μg
638 ng/μl 1.91 191.4 μg




Figure 2. Gel electrophoresis analysis of 10 kb plasmid DNA purified from 40 ml culture using
GeneMark Plasmid Midiprep Purification Kit (DP01MD) and with related kit from supplier Q.
M: GenKB DNA Ladder | Q: Using related kit from Supplier Q | GM: GeneMark Plasmid Midiprep.

Supplier Final Concentration A260/A280 Yield Average
Supplier Q 358 ng/µl 1.85 107.4 µg 97.6 µg
266 ng/µl 1.87 79.8 µg
352 ng/µl 1.88 105.6 µg

GeneMark
458 ng/μl 1.87 137.4 μg 115.2 μg
374 ng/μl 1.89 112.2 μg
338 ng/μl 1.88 101.4 μg
366 ng/μl 1.89 109.8 μg
General Procedure


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