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PLV-10011-50

Krueppel-like factor 4 (KLF4) is a zinc finger transcriptional regulator that has been identified as a good indicator of stem cell-like capacity and is subsequently used in iPS cell generation. KFL4 plays an important role in maintaining stem cells, and in preventing their differentiation. 

Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts. EF-1 alpha (or EF1a) is often useful in conditions where other promoters (such as CMV) have diminished activity or have been silenced (as in embryonic stem cells).

Virus: EF1A-KLF4
Titer (approx): 1 x 108 CFU/ml
Vector Information: Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for Target Gene: EF1A
Target Gene / Reporter(s): KLF4
Selection Gene: Puromycin
Biosafety Level: BSL-2
Shipped: Dry ice
Storage: Store at -80°C


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