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NANOG is a transcription factor important for maintaining the self-renewal capacity of undifferentiated stem cells. Ectopic expression of NANOG has been used to generate iPS cells in culture.
Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts.EF-1 alpha (or EF1a) is often useful in conditions where other promoters (such as CMV) have diminished activity or have been silenced (as in embryonic stem cells).
| Virus: | EF1A-NANOG_m |
| Titer (approx): | 1 x 108 CFU/ml |
| Vector Information: | Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) |
| Promoter for Target Gene: | EF1A |
| Target Gene / Reporter(s): | human NANOG |
| Selection Gene: | Puromycin |
| Biosafety Level: | BSL-2 |
| Shipped: | Dry ice |
| Storage: | Store at -80°C |
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