OCT3/4 (POU5F1) is a transcription factor that is expressed in stem cells and germ cells. Its expression is required to sustain cell self-renewal of undifferentiated stem cells. Oct-3/4 is the most recognized marker for totipotent ES cells. Ectopic expression of OCT3/4 has been used to produce iPS cells in culture.
Human elongation factor-1 alpha (EF-1 alpha) is a constitutive promoter of human origin that can be used to drive ectopic gene expression in various in vitro and in vivo contexts. EF-1 alpha (or EF1a) is often useful in conditions where other promoters (such as CMV) have diminished activity or have been silenced (as in embryonic stem cells).
Virus: |
EF1A-OCT3/4 |
Titer (approx): |
1 x 108 CFU/ml |
Vector Information: |
Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) |
Promoter for Target Gene: |
EF1A |
Target Gene / Reporter(s): |
OCT3/4 |
Selection Gene: |
Puromycin |
Biosafety Level: |
BSL-2 |
Shipped: |
Dry ice |
Storage: |
Store at -80°C |