Deoxyribonuclease I (DNase I; EC126.96.36.199) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.1,2 The catalytic activity of DNase I entails an obligate requirement for Ca2+ as its metal cofactor while Mg2+ ions offer a synergistic component to the reaction milieu.3 DNase I is synonymous with Pancreatic DNase, Endodeoxyribonuclease I and Thymonuclease.
- Degrade template DNA following in vitro transcription
- Remove contaminating genomic DNA from protein samples
- Mediate nick translation
- Reduce viscosity of cell lysates and prevent clumping when handling cultured cells
- Mediate DNase I foot-printing
2mM CaCl2,10mM Tris-HCl (pH 7.6) and 50% glycerol. Store at -20°C.
Shipped with dry ice
(1) Kunitz, M (1950). J. Gen. Physiol. 33, 349-362.
(2) Vanecko, S and Laskowski, M (1961). J Biol Chem 236: 3312-3316.
(3) Moores, S (1981). Pancreatic DNase, in: The Enzymes (P.D. Boyer, Ed.) Academic Press, New York, Chapter 15.
One unit is defined as the amount of enzyme which will completely degrade 1μg of pBR322 DNA in 10 mins at 37 °C. Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.