DNase I 2000U (1.0ml)

Description	Deoxyribonuclease I (DNase I; EC3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.^1,2 The catalytic activity of DNase I entails an obligate requirement for Ca²⁺ as its metal cofactor while Mg²⁺ ions offer a synergistic component to the reaction milieu.³ DNase I is synonymous with Pancreatic DNase, Endodeoxyribonuclease I and Thymonuclease.
Manual
Application	- Degrade template DNA following in vitro transcription - Remove contaminating genomic DNA from protein samples - Mediate nick translation - Reduce viscosity of cell lysates and prevent clumping when handling cultured cells - Mediate DNase I foot-printing
Storage	2mM CaCl₂,10mM Tris-HCl (pH 7.6) and 50% glycerol. Store at -20°C.
Handling	Shipped with dry ice
Reference	(1) Kunitz, M (1950). J. Gen. Physiol. 33, 349-362. (2) Vanecko, S and Laskowski, M (1961). J Biol Chem 236: 3312-3316. (3) Moores, S (1981). Pancreatic DNase, in: The Enzymes (P.D. Boyer, Ed.) Academic Press, New York, Chapter 15.
Notes	One unit is defined as the amount of enzyme which will completely degrade 1μg of pBR322 DNA in 10 mins at 37 °C. Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller.

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