Protease Inhibitor Cocktail is used to prevent the digestion of proteins that occurs after cell lysis takes place. The presence of EDTA ensures greater inhibition of metalloproteases.
100mM PMSF, 100mM EDTA, 2mM Bestatin, 0.3mM Pepstatin A, and 0.3mM E-64
1mL of the Protesase Inhibitor Cocktail is recommended for the inhibition of protease activity form 100mL of cell lysate at a density of 10^8 cells per mL.
However, since not all organisms contain the same level of endogenous proteases, it is up to the researcher to optimize the concentration for their experiments.
Store cocktail at -20°C. Cocktail is dissolved in DMSO containing little deionized water. The cocktail may form crystals. If there are crystals, place it at RT for 5 minutes. The cocktail is stable for a year.
As this item contains PMSF, please operate carefully. PMSF is toxic and causes irritation to the eyes and skin. Wear eye and hand protection and proper lab attire when handling the item.