Description One reaction tube for RT and PCR now offers unparalleled sensitivity and efficiency. With abm’s One-Step RT-PCR Kit, you can streamline the procedure for reverse transcription and subsequent PCR in the same reaction tube, eliminating the need to change buffers and reducing the risk of contamination. This kit offers the end-user an efficient and easy alternative to the conventional “two-step” RT-PCR approach with extreme sensitivity, specificity and high product yield. 

This MasterMix contains Cresol Red as an electrophoresis dye, with migration equivalent to 125 bp DNA fragment on an agarose gel.
Literature Click To Open
Application • Gene-expression analysis
• Transcription analysis
• Gene cloning
• Multiplex RT-PCR
• High throughput applications
• Virus detection and quantification
Protocol Click To Open
Storage Store all components at -20°C.
Notes • Multiple freezing and thawing of RNA should be avoided. Thaw and keep RNA on ice.
• It is recommended that the One-Step RT-PCR is setup under conditions where RNase contamination has been eliminated. Pipette tips and tubes should be treated with 0.1% DEPC.
• Wearing gloves when performing procedure is highly recommended.

This product is distributed for laboratory research only. Caution: Not for diagnostic use

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