OnePCRTM is a ready-to-use PCR reaction mixture. By simply adding primers, template, and water, the reagent will execute primer extensions and other molecular biology applications.. OnePCRTM is a pre-mixed solution containing Taq DNA polymerase, PCR Buffer, dNTPs, gel loading dyes,and Novel Green. OnePCRTM, which contains the fluorescence dye – Novel Green, is excited at 497 nm but also shows a secondary excitation peak at 248 nm (Fig.1a). After bound to DNA, the fluorescent emission of the Novel Green is centered at 524 nm (Fig. 1b). These spectral characteristics enable this fluorescent dye to be compatible with a wide variety of gel reading facilities, including UV epi- and transilluminator, argon laser and mercury-arc lamp excitation gel scanners. OnePCRTM mixture is supplied at the 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents are provided with the sufficient amplification reactions of 50 μl each.
No post-staining procession.
No need to prepare PCR reagents.
Direct loading onto your agarose gel for analysis.
Sensitivity- High degree of sensitivity as the ethium bromide.
Time Efficiency- No de-staining requirement, low background value, and image displayed after coupling with the nucleic acid.
Compatibility – Use the Blue Light or UV to detect the signal.