TaqProbe 2x qPCR Mastermix-iCycler 4 X 1.25 ml, for 500 reactions (20 µl)

Description	Ultimate sensitivity in real-time PCR with abm’s TaqProbe 2X qPCR MasterMix. abm’s TaqProbe 2X qPCR MasterMix is designed for high throughput quantitative PCR using TaqMan® probe-based chemistry. Available with the option of ROX or fluorescein as the internal passive reference dye, abm’s TaqProbe 2X qPCR MasterMix offers superb performance in sensitivity and signal-to-noise ratio. The multiplex formulation supports quantitative amplification and detection of up to four targets simultaneously with consistent and reliable results. Key Features • Enable streamlined protocol in a simple reaction set-up • Allow accurate quantification of a variety of gene targets • Reduce pipetting steps to minimize the risk of contamination • Compatible with most real-time PCR instruments Please refer to our EvaGreen qPCR MasterMix Selection Guide in selecting the appropriate qPCR formulation applicable to your particular instrument model.
Literature
Application	• Gene-expression analysis • Gene knockdown validation • SNP genotyping assays • CHiP • Copy number variation • Microarray validation • High throughput applications • Virus detection and quantification
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Protocol
Storage	Stored at -20°C and protect from light.
Notes	1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles. 2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results. 3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range. This product is distributed for laboratory research only. Caution: Not for diagnostic use.

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