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DescriptionUltimate sensitivity in real-time PCR with abm’s TaqProbe 2X qPCR MasterMix. abm’s TaqProbe 2X qPCR MasterMix is designed for high throughput quantitative PCR using TaqMan® probe-based chemistry. Available with the option of ROX or fluorescein as the internal passive reference dye, abm’s TaqProbe 2X qPCR MasterMix offers superb performance in sensitivity and signal-to-noise ratio. The multiplex formulation supports quantitative amplification and detection of up to four targets simultaneously with consistent and reliable results.

Key Features

• Enable streamlined protocol in a simple reaction set-up
• Allow accurate quantification of a variety of gene targets
• Reduce pipetting steps to minimize the risk of contamination
• Compatible with most real-time PCR instruments

Please refer to our qPCR MasterMix Selection Guide (found in theDocuments section below) for selecting the appropriate qPCR formulation applicable to your particular instrument model.LiteratureClick To Open
Application
Gene-expression analysis
• Gene knockdown validation 
• SNP genotyping assays 
• CHiP
• Copy number variation
• Microarray validation
• High throughput applications
• Virus detection and quantificationPicture 1ProtocolClick To OpenStorageStored at -20°C and protect from light.Notes1. Aliquot reagents to avoid contamination and to avoid repeated freeze-thaw cycles.
2. Primer concentration should not be high, a concentration of 100 nM to 300 nM of each primer usually gives the best results.
3. A very effective way to get tight Ct among replicates is to reduce pipetting error, this can be achieved by: preparing amplicon specific pre-mix, using repeating pipet, and keeping pipetting volume in manufacture suggested range.
4. For optimal results, it is recommended that the primers are 18-22 nucleotides in length with a Tm of 58°C-60°C and the size of target is about 100-250 bp.

This product is distributed for laboratory research only. 
Caution: Not for diagnostic use.


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