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The EasyPrep™ Plasmid Miniprep Kit provides a simple, fast and cost effective method to purify plasmid DNAs (10-30 µg) from small volumes of bacterial culture (1-5 ml), which is typical in bacterial colony screening during plasmid construction. The kit applies DNA-binding specific matrix, which allows the high efficient binding of DNA, while proteins and other cellular components are easily removed during washing. Plasmid DNAs are easily eluted with Tris buffer or pure water.
Plasmid DNAs purified from EasyPrep™ plasmid miniprep kit can be used directly for downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.
Kit's content:
|
Catalog #
|
PD01-01
|
PD01-02
|
|
Preps
|
50
|
250
|
| DNA Columns |
50
|
250
|
| Buffer A1 |
20 ml
|
80 ml
|
| Buffer A2 |
20 ml
|
80 ml
|
| Buffer N1 |
25 ml
|
110 ml
|
| DNA Wash Buffer* |
12 ml
|
50 ml
|
| Elution Buffer |
10 ml
|
20 ml
|
| Column Buffer | 10 ml | 30 ml |
| RNase A |
70 µl
|
280 µl
|
| Manual | 1 | 1 |
* Add ethanol to washing buffer before use
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