Cre Recombinase is a tyrosine recombinase enzyme derived from the P1 Bacteriophage. The enzyme uses a topoisomerase I like mechanism to carry out site specific recombination events. The enzyme (38kDa) is a member of the Integrase family of site specific recombinase and it is known to catalyse the site specific recombination event between two DNA recognition sites (loxP sites). This premade lentivirus expresses Cre Recombinase under the control of PGK promoter, and Firefly luciferase reporter under the control of CMV promoter.
Virus:
PGK-Cre with CMV-Firefly luciferase reporter
Titer (approx):
1 x 108 TU/ml
Vector Information:
Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)