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Inducible tet-on PAX6 lentivirus

Pax6 gene contains a paired domain and a paired-type homeodomain and is expressed during early neuroretina development. Pax6 is required for multipotency of retinal progenitors. Tansfection of Pax6 into ES or iPS cells induces retinal progenitors. Pax6 alone is sufficient to induce transdifferentiation of ectopic neural retina (NR) from retinal pigment epithelial (RPE) cells. This lentivirus expresses human Pax6 under the control of the Tet promoter.

Virus: Inducible tet-on PAX6
Titer (approx): 1 x 108 CFU/ml
Vector Information: Vector includes both 5’ and 3’ lentiviral LTR and all necessary elements for effective transduction; woodchuck hepatitis virus posttranscriptional regulatory element (WPRE)
Promoter for Target Gene: TRE
Target Gene / Reporter(s): PAX6
Selection Gene: Puromycin
Biosafety Level: BSL-2
Shipped: Dry ice
Storage: Store at -80°C


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