GenJet™ DNA In Vitro Tranfection Reagent for Hela is pre-optimized for Hela cell transfection. Refer to the following optimal transfection conditions for maximal transfection efficiency on Hela cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium 5 Hours After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Efficiency (GFP %)

DMEM with 4.5 g/L glucose, 10% FBS
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
48 hours

pEGFP-N3 (CMV promoter) 
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

A Picture Showing Transfection Efficiency of GenJet™ Reagent on Hela Cells

GenJet™ reagent is optimized for Hela cell (ATCC # CCL-2.2). Hela cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 48 hours post transfection 

Data Sheet 

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