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GenJet™ DNA In Vitro Tranfection Reagent for Hela is pre-optimized for Hela cell transfection. Refer to the following optimal transfection conditions for maximal transfection efficiency on Hela cells. GenJet™ reagent, 1.0 ml, is sufficient for 600 to 1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium 5 Hours After Transfection Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
~80% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes 48 hours EGFP pEGFP-N3 (CMV promoter) 88% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet™ Reagent on Hela Cells
GenJet™ reagent is optimized for Hela cell (ATCC # CCL-2.2). Hela cells were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by GenJet™. The efficiency was checked 48 hours post transfection
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