GenJet™ DNA In Vitro Tranfection Reagent for Jurkat cell is pre-optimized for transfecting Jurkat cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on Jurkat cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Efficiency (GFP %)

PRMI with 10% FBS
1xGenJet™ Transfection Buffer
15 minutes at RT
48 hours

pEGFP-N3 (CMV promoter) 

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet & Protocol 

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