Contents Per Kit:
● 1x1.0 ml of GenJet™ DNA Transfection Reagent
● 1x1.0 ml (x50) of HapiCell™ Transfection Toxicity Removal Cocktail
Description
GenJet™ DNA In Vitro Tranfection Reagent for keratinocytes is pre-optimized for transfecting human or mouse primary or immortalized keratinocytes. Keratinocytes are hard to transfect partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 50% efficiency with minimal cell death.
Refer to the following optimal transfection conditions for maximal transfection efficiency on keratinocytes. GenJet™ reagent, 1.0 ml, is sufficient for ~600 transfections in 24 well plates or ~300 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Treatment with HapiCell™ Toxicity Removal Cocktail
Maximal Efficiency
Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)
|
90%
DMEM with 4.5 g/L glucose, 10% FBS
3:1
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
Yes, ~5 Hours After Transfection Followed by PBS Wash
48 hours
EGFP
pEGFP-N3 (CMV promoter)
56%
|
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet 