Description
GenJet™ DNA In Vitro Tranfection Reagent for PC-3 is pre-optimized for transfecting PC-3 cells.  This human prostate cancer cell line very sensitive to liposome based mediated toxicity, leading high percentage of cell death.  With the pre-optimized DNA transfection reagent, up to 60% efficiency can be achieved. 
Refer to the following optimal transfection conditions for maximal transfection efficiency on PC-3 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
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	 Summary of Optimal Transfection Conditions: 
	Confluence on the day of transfection 
	Cell culture conditions 
	GenJet™ (µl) : DNA (µg) Ratio 
	Diluent for DNA and Transfection Reagent 
	Incubation Time to Form GenJet™/DNA Complex 
	Presence of Serum/Antibiotics during Transfection 
	Change Medium After Transfection 
	Maximal Efficiency  
	 
	Transfection Results: 
	Reporter Gene 
	Plasmid 
	Efficiency (GFP %) 
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	90% 
	DMEM with 4.5 g/L glucose, 10% FBS 
	3:1 
	Serum-free DMEM with 4.5 g/L glucose 
	15 minutes at RT 
	Yes 
	Yes, ~5 Hours After Transfection 
	48 hours 
	 
	 
	EGFP 
	pEGFP-N3 (CMV promoter)  
	60% 
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Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet 