Description

GenJet™ DNA In Vitro Tranfection Reagent for SKOV3 cell is pre-optimized for transfecting SKOV3 cells

Refer to the following optimal transfection conditions for maximal transfection efficiency on SKOV3 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency 


Transfection Results:
Reporter Gene
Plasmid
Efficiency (GFP %)


90%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes, 18~24 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter) 

82%


Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet 


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