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● 1x1.0 ml of GenJet™ DNA Transfection Reagent
● 1x1.0 ml (x50) of HapiCell™ Transfection Toxicity Removal Cocktail
Description
GenJet™ DNA In Vitro Tranfection Reagent for SMC is pre-optimized for transfecting smooth muscle cells. Smooth muscle cells are notoriously hard to transfect partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 75% efficiency.
Refer to the following optimal transfection conditions for maximal transfection efficiency on Hela cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
| Summary of Optimal Transfection Conditions: Confluence on the day of transfection Cell culture conditions GenJet™ (µl) : DNA (µg) Ratio Diluent for DNA and Transfection Reagent Incubation Time to Form GenJet™/DNA Complex Presence of Serum/Antibiotics during Transfection Change Medium After Transfection Treatment with HapiCell™ Toxicity Removal Cocktail Maximal Efficiency Transfection Results: Reporter Gene Plasmid Efficiency (GFP %) |
95% DMEM with 4.5 g/L glucose, 10% FBS 3:1 Serum-free DMEM with 4.5 g/L glucose 15 minutes at RT Yes Yes, ~5 Hours After Transfection Yes, ~5 Hours After Transfection Followed by PBS Wash 48 hours EGFP pEGFP-N3 (CMV promoter) 55% |
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
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