Contents Per Kit:
● 1x1.0 ml of GenJet™ DNA Transfection Reagent
● 1x1.0 ml (x50) of HapiCell™ Transfection Toxicity Removal Cocktail

GenJet™ DNA In Vitro Tranfection Reagent for SMC is pre-optimized for transfecting smooth muscle cells. Smooth muscle cells are notoriously hard to transfect partly due to the transfection toxicity related cell death. In combination of our proprietary HapiCell™ Toxicity Removal Cocktail, the pre-optimized transfection reagent gave up to 75% efficiency. 

Refer to the following optimal transfection conditions for maximal transfection efficiency on Hela cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent
Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Treatment with HapiCell™ Toxicity Removal Cocktail
Maximal Efficiency 

Transfection Results:
Reporter Gene
Efficiency (GFP %)

DMEM with 4.5 g/L glucose, 10% FBS
Serum-free DMEM with 4.5 g/L glucose
15 minutes at RT
Yes, ~5 Hours After Transfection
Yes, ~5 Hours After Transfection Followed by PBS Wash
48 hours

pEGFP-N3 (CMV promoter) 
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer

Data Sheet 

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