GenMute™ siRNA Transfection Reagent for Primary Keratinocytes is pre-optimized for transfecting siRNA to primary keratinocytes. Primary keratinocytes are known for notorious resistance for DNA and siRNA transfections. With the pre-optimized transfection conditions, average 90% silencing can be obtained.
Size & content:
- GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate
- GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution
Refer to the following optimal transfection conditions for maximal silencing on primary keratinocytes. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency
Silencing Efficiency (% )
DMEM with 4.5 g/L glucose, 10% FBS
1xGenMute™ Transfection Buffer
~15 minutes at RT
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Data Sheet & Protocol