GenMute™ siRNA Transfection Reagent for MEFs is pre-optimized for transfecting siRNA to Mouse Embryonic Fibroblasts (MEFs). MEFs are required to support the growth of undifferentiated mouse or human ES cells, iPS cells, thus they are also called feeder cells. MEFs are isolated from mouse embryos and are used at their early passages. Before MEFs are used as feeder cells, they must be treated by irradiation or mitomycin C to stop the cells from further dividing.
Refer to the following optimal transfection conditions for maximal silencing on MEFs. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency
Silencing Efficiency (% )
DMEM with 4.5 g/L glucose, 10% FBS
1xGenMute™ Transfection Buffer
~15 minutes at RT
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Data Sheet & Protocol