GenMute™ siRNA Transfection Reagent for SMC is pre-optimized for transfecting siRNA to smooth muscle cells (SMC).
Refer to the following optimal transfection conditions for maximal silencing on SMC. GenMute™ reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or ~416 transfections in 6 well plates.
Summary of Optimal Transfection Conditions:
Cell culture medium
Confluency of cell on the day of transfection
Optimal siRNA concentration
Diluent for siRNA and GenMute™ Reagent
Incubation Time to Form GenMute™/siRNA Complex
Maximal Silencing Efficiency
Silencing Efficiency (% )
DMEM with 4.5 g/L glucose, 10% FBS
1xGenMute™ Transfection Buffer
~15 minutes at RT
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
Data Sheet & Protocol