Description: 
This recombinant human adenovirus type 5 expresses a codon-optimized Cas9 nuclease fusion with GFP as reporter under CMV promote and can be used along with guided RNA for genome-editing.

The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering.  Cas9 (CRISPR associated protein 9) is an RNA-guided DNA nuclease enzyme associated with Streptococcus pyogenes' CRISPR immunity system.  The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms.

Product Category: human Adenovirus Type5 (dE1/E3). Ad-CMV-Cas9-GFP

Titer: 1E+10 ~ 1E+11 PFU/ml 

Storage Buffer: DMEM with 2% BSA & 2.5% Glycerol

User Manual: