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Superexpress 2000 Transfection Reagent is fomulated with proprietary cationic polymer to ensure low cytotoxicity and high transfection efficiency. Superexpress 2000 was shown to efficiently deliver genes to various established cell lines as well as primary cells, which include HEK293, 293T, 293E, CHO, COS1, HeLa, NIH 3T3, insect cell lines (Sf9 and Sf21) and a variety of other eucaryotic cell lines with low cytotoxicity. Superexpress 2000 reagent, 1.0ml, is sufficient for 300 to 600 transfections in 24 well plates, or 150 to 300 transfections in 6 well plates.
Features
- Efficient for difficult-to-transfect cells
- Transfect a broad range of mammalian cells
- Low cytotoxicity
- No sera interference
- Compatible with Lipofectamine 2000 protocol
- Simple protocol
- Suitable for high-thoughput application
Storage conditions
Upon arival store this product at -200 C or 40C. If stored properly, the product will be stable for 12 months or longer. Product shipped at ambient temperature.
Fig. A comparision of transfection efficiency of Superexpress (#5 & #6) with other popular transfection reagents (#1-#4, #7, #8). Amount of transfection reagents: 3μl; 1μg DNA; 293 cells grown and a 12-well plate
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